However, some of this advantage is lost if the feed is a mixture of BSA and Tg since, in this case, Tg binding leads to greater diffusional hindrance for BSA.Ĭore-shell resins Diffusional hindrance Dynamic binding capacity Flow-through purification Modeling.Ĭopyright © 2021. Column measurements show that, despite the higher static capacity of Capto Core 400 for BSA, the dynamic binding capacity is greater for Capto Core 700 as a result of its faster kinetics. Adsorbed Tg further hinders diffusion of BSA in both resins. These values decrease dramatically for Tg to 0.022 × 10 -7 and 0.088 × 10 -7 cm 2/s and to 0.13 × 10 -7 and 0.59 × 10 -7 cm 2/s for Capto Core 400 and 700, respectively. Shop Cytiva Capto Core 700 at Fishersci. For BSA, core and shell effective pore diffusivities are about 0.25 × 10 -7 and 0.6 × 10 -7 cm 2/s, respectively, for Capto Core 400, and about 1.6 × 10 -7 and 2.6 × 10 -7 cm 2/s, respectively, for Capto Core 700. Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules. Mass transfer in both resins is affected by diffusional resistances through the shell and within the adsorbing core. However, for the much larger Tg, the attainable capacity is substantially larger for Capto Core 700. Because of the smaller pores and higher surface area, the BSA binding capacity of Capto Core 400 is approximately double that of Capto Core 700. Capto Core 700 is a core-shell chromatographic support with an adsorbing core contained within an inert shell layer designed to purify larger biomolecules. Although shell thicknesses are comparable (3.6 and 4.2 µm for Capto Core 400 and 700, respectively), the two resins differ substantially in pore size (pore radii of 19 and 50 nm, respectively). Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules. Both resins are agarose-based and contain an adsorbing core surrounded by an inert shell. Capto Core 700 is also available as a bulk medium in a range of pack sizes from lab scale to production-scale.Structural and functional characteristics of the two core-shell resins Capto™ Core 400 and 700, which are useful for the flow-through purification of bioparticles such as viruses, viral vectors, and vaccines, are compared using bovine serum albumin (BSA) and thyroglobulin (Tg) as models for small and large protein contaminants. The prepacked HiTrap™ and HiScreen™ formats simplify process development and scale-up. With Capto Core 700, flowthrough chromatography and a wide window of operation enable straightforward optimization. Smaller impurities bind to the internalized ligands, which are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The inactive outer layer excludes large molecules (Mr 700 000 and above) from entering the beads which can subsequently be collected in the column flowthrough. Storage and Stability Store at 4 to 30 ☌ (20 Ethanol) Analysis Note To view the Certificate of Analysis for this product, please visit. The ligand is functional in sodium phosphate and Tris buffers containing up to 1 M NaCl (Fig 4). This chromatography resin has both size exclusion and binding properties. The octylamine ligand chosen for Capto Core 400 and Capto Core 700 is multimodal, giving a broad window of operation with excellent binding capacity in a range of buffers. The Capto Core 700 medium employs core bead technology, with a ligand-activated core and inactive outer layer. General description Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality. Optimization and scale-up are both straightforward. Productivity is improved with up to 100-fold greater sample loadings and significantly higher flow rates. Capto Core 700 offers a range of performance advantages over a typical gel filtration step. GE Healthcare’s Capto Core 700 is a multimodal chromatography medium (resin) designed for intermediate purification and polishing of viruses and other large biomolecules.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |